Moving the lever of the diaphragm permits a greater or smaller amount of light to come through the opening of the stage. Look through the eyepiece and switch on the lamp or adjust the mirror so that a circle of light can be seen. A click indicates that the lens is in line with the opening of the stage. Rotate the nosepiece so that the low-power objective (10×) is directly in line with the body tube. Raise the body tube by turning the coarse adjustment knob until the objective lens is about 2 cm above the opening of the stage.

The microscope's base should be resting evenly on the table, approximately 10 cm from the table's edge. Gently place the microscope on the lab table with the arm facing you. Use the following procedures when working with the microscope.Ĭarry the microscope by placing one hand beneath the base and grasping the arm of the microscope with the other hand. Learning the name, function, and location of each of the microscope's parts is necessary for proper use. For example, with a 10× eyepiece and a 40× objective, the total magnification is 10 × 40 = 400×. To determine the total magnification, multiply the magnifying power of the eyepiece by the magnifying power of the objective.

The magnification is marked on the objective. The longer ones are of high power, usually up to 40× or 43×. The shortest objective is a low-power magnifier, usually 10×. By rotating the nosepiece, you can select the objective through which you will view your specimen. Other lenses, called objective lenses, are at the bottom of the body tube on the revolving nosepiece.

This lens usually has a magnification of 10×. The eyepiece lens is located in the top portion of the microscope. The microscope used in most biology classes, the compound microscope, contains a combination of lenses.

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